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M-MLV Reverse Transcriptase (RNase H-)
售價 NT$ 2,500
● Package: 10,000U (200 u/μl)
● MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus
(MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the
complementary cDNA first strand from a single-stranded RNA template to
which a primer has been hybridized. MMLV RT will also extend primers
hybridized to single-stranded DNA. Second strand cDNA synthesis can be
achieved from some mRNA templates without an additional DNA
polymerase. M-MLV RT (H- ) can synthesized 9.5-kb products, the largest
RNA component in the reaction. However, M-MLV RT synthesized more full-
length cDNA regardless of size.
● Unit definition:One unit of activity is the amount of enzyme required to
incorporate 1 nmole of dTTP into an acid-insoluble form in 10 minutes at
37°C using polyA-oligo(dT) as template and primer.
● MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus
(MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the
complementary cDNA first strand from a single-stranded RNA template to
which a primer has been hybridized. MMLV RT will also extend primers
hybridized to single-stranded DNA. Second strand cDNA synthesis can be
achieved from some mRNA templates without an additional DNA
polymerase. M-MLV RT (H- ) can synthesized 9.5-kb products, the largest
RNA component in the reaction. However, M-MLV RT synthesized more full-
length cDNA regardless of size.
● Unit definition:One unit of activity is the amount of enzyme required to
incorporate 1 nmole of dTTP into an acid-insoluble form in 10 minutes at
37°C using polyA-oligo(dT) as template and primer.
● Storage conditions: -20°C
